The College will address fundamental and experimental aspects of advanced Microscopy, Spectroscopy and related techniques. The scope of the course is to promote new theoretical and experimental methods, concepts, instruments, measurement techniques and data analysis routines for both Laboratory and industrial applications to train students and scientists, as well as to coordinate international activities and collaborations in this area. By focusing on both theory and applications, the College will also provide an interesting intersection of emerging techniques and experimental methods with theoretical advances in the field. The lectures will focus on a variety of topics related to biological applications, environmental research and material characterization. Hands-on sessions will be organized every day of the college in the ICTP laboratories accompanied by library documentation finalized with round-table discussion.